| 质粒名称: | pcDNA3.1_ spike_del19 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 155297 |
| 质粒用途: | Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles |
| 存储实验室: | Raffaele De Francesco |
|
载体骨架基本信息 |
|
| 载体名称: | pCDNA3.1 |
| 载体类型: | Generation of SARS-CoV-2-pseudotyped lentiviral particles |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-Cov2 spike_deleted |
| 物种来源: | SARS-Cov2 |
| 基因突变: | Deletion in C-term 19 aa of spike |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NheI（销毁后未知） |
| 3’克隆位点: | XhoI（销毁后未知） |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1_ spike_del19 was a gift from Raffaele De Francesco (Addgene plasmid # 155297 ; http://n2t.net/addgene:155297 ; RRID:Addgene_155297) |
| 参考文献部分: | COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models. Conforti A, Marra E, Palombo F, Roscilli G, Rava M, Fumagalli V, Muzi A, Maffei M, Luberto L, Lione L, Salvatori E, Compagnone M, Pinto E, Pavoni E, Bucci F, Vitagliano G, Stoppoloni D, Pacello ML, Cappelletti M, Ferrara FF, D'Acunto E, Chiarini V, Arriga R, Nyska A, Di Lucia P, Marotta D, Bono E, Giustini L, Sala E, Perucchini C, Paterson J, Ryan KA, Challis AR, Matusali G, Colavita F, Caselli G, Criscuolo E, Clementi N, Mancini N, Gross R, Seidel A, Wettstein L, Munch J, Donnici L, Conti M, De Francesco R, Kuka M, Ciliberto G, Castilletti C, Capobianchi MR, Ippolito G, Guidotti LG, Rovati L, Iannacone M, Aurisicchio L. Mol Ther. 2021 Sep 20. pii: S1525-0016(21)00466-4. doi: 10.1016/j.ymthe.2021.09.011. 10.1016/j.ymthe.2021.09.011 PubMed 34547465 |
pcDNA3.1_ spike_del19
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规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
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买家导航
pcDNA3.1_ spike_del19载体质粒基本信息
pcDNA3.1_ spike_del19质粒图谱载体图谱和pcDNA3.1_ spike_del19载体序列质粒序列多克隆位点信息
pcDNA3.1_ spike_del19质粒载体简介
pcDNA3.1_ spike_del19质粒序列载体序列
LOCUS pcDNA3.1_ spike_del19 9118 bp DNA circular SYN 04-SEP-2026
DEFINITION Expression of spike with C-term deletion of 19 aa, used to generate
high efficiency SARS-CoV-2-pseudotyped lentiviral particles.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9118)
AUTHORS Conforti A, Marra E, Palombo F, Roscilli G, Rava M, Fumagalli V,
Muzi A, Maffei M, Luberto L, Lione L, Salvatori E, Compagnone M,
Pinto E, Pavoni E, Bucci F, Vitagliano G, Stoppoloni D, Pacello ML,
Cappelletti M, Ferrara FF, D'Acunto E, Chiarini V, Arriga R, Nyska
A, Di Lucia P, Marotta D, Bono E, Giustini L, Sala E, Perucchini C,
Paterson J, Ryan KA, Challis AR, Matusali G, Colavita F, Caselli G,
Criscuolo E, Clementi N, Mancini N, Gross R, Seidel A, Wettstein L,
Munch J, Donnici L, Conti M, De Francesco R, Kuka M, Ciliberto G,
Castilletti C, Capobianchi MR, Ippolito G, Guidotti LG, Rovati L,
Iannacone M, Aurisicchio L
TITLE COVID-eVax, an electroporated DNA vaccine candidate encoding the
SARS-CoV-2 RBD, elicits protective responses in animal models.
JOURNAL Mol Ther. 2021 Sep 20. pii: S1525-0016(21)00466-4. doi:
10.1016/j.ymthe.2021.09.011.
PUBMED 34547465
REFERENCE 2 (bases 1 to 9118)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 4, 2026 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther.
2021 Sep 20. pii: S1525-0016(21)00466-4. doi:
10.1016/j.ymthe.2021.09.011."
COMMENT Sequence Label: pcDNA3.1_ spike_del19
FEATURES Location/Qualifiers
source 1..9118
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 629..648
/label=T7
/note="T7 promoter, forward primer"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4475..4492)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4481..4705
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4751..5179
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4838..4857)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5048..5069
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5188..5208)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5193..5522
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5373..5508
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5435..5454
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5589..6383
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5643..5662)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6253..6272
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6557..6678
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6594..6613)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6648..6667
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6727..6743)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6727..6743)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6740..6762)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6751..6767
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(6775..6781,6782..6799,6800..6805))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6820..6841
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6958..6975)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7129..7717)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7209..7228)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(7888..8679,8680..8748))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8511..8530
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8749..8853)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8928..8947)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
661 gctagcatgt ttgtcttcct ggtcctgctg cctctggtct cgtctcagtg cgtgaacctg
721 actactagaa cccagctgcc tcctgcctat actaactcct tcacccgcgg cgtgtactac
781 ccagacaagg tgttccgcag ctccgtgctg cactccaccc aggatctgtt cctgcccttc
841 ttcagcaacg tgacctggtt ccacgccatc cacgtgagcg gcaccaatgg caccaagcgg
901 ttcgacaatc ccgtgctgcc attcaacgat ggcgtgtact tcgcctccac cgagaagagc
961 aacatcatcc gcggctggat cttcggcacc accctggact ccaagaccca gagcctgctg
1021 atcgtgaaca atgccaccaa cgtggtcatc aaggtgtgcg agttccagtt ctgcaatgat
1081 ccattcctgg gcgtgtacta ccacaagaac aataagtcct ggatggagag cgagttccgc
1141 gtgtacagct ccgccaacaa ttgcaccttc gagtacgtgt cccagccctt cctgatggac
1201 ctggagggca agcagggcaa tttcaagaac ctgcgcgagt tcgtgttcaa gaatatcgat
1261 ggctacttca agatctactc caagcacacc cccatcaacc tggtgcgcga cctgccacag
1321 ggcttcagcg ccctggagcc actggtggat ctgccaatcg gcatcaacat caccaggttc
1381 cagaccctgc tggccctgca ccgcagctac ctgaccccag gcgacagctc cagcggatgg
1441 accgctggag ctgctgccta ctacgtgggc tacctgcagc cccgcacctt cctgctgaag
1501 tacaacgaga atggcaccat caccgacgcc gtggattgcg ccctggatcc actgtccgag
1561 acaaagtgca ccctgaagag cttcaccgtg gagaagggca tctaccagac ctccaatttc
1621 cgcgtgcagc caaccgagag catcgtgcgc ttccccaata tcaccaacct gtgcccattc
1681 ggcgaggtgt tcaacgctac caggttcgcc agcgtgtacg cttggaatcg caagcgcatc
1741 tccaactgcg tggccgacta cagcgtgctg tacaactccg ccagcttctc caccttcaag
1801 tgctacggcg tgtcccccac caagctgaat gatctgtgct tcaccaacgt gtacgccgat
1861 agcttcgtga tcaggggcga cgaggtgcgc cagatcgctc caggacagac cggcaagatc
1921 gctgactaca attacaagct gcccgacgat ttcaccggct gcgtgatcgc ctggaactcc
1981 aacaatctgg atagcaaagt gggcggcaac tacaattacc tgtaccgcct gttccgcaag
2041 tccaatctga agccattcga gcgcgacatc tccaccgaga tctaccaggc tggaagcacc
2101 ccatgcaatg gagtggaggg cttcaactgc tacttccccc tgcagagcta cggcttccag
2161 ccaaccaacg gagtgggata ccagccatac agggtggtgg tgctgtcctt cgagctgctg
2221 cacgctccag ctaccgtgtg cggaccaaag aagagcacca atctggtgaa gaacaagtgc
2281 gtgaacttca atttcaacgg cctgaccgga accggcgtgc tgaccgagtc caacaagaag
2341 ttcctgccat tccagcagtt cggaagggac atcgctgata ccaccgacgc cgtgcgcgac
2401 ccacagaccc tggagatcct ggatatcacc ccatgctcct tcggcggcgt gagcgtgatc
2461 accccaggaa ccaataccag caaccaggtg gccgtgctgt accaggacgt gaattgcacc
2521 gaggtgccag tggctatcca cgctgatcag ctgaccccaa cctggcgcgt gtacagcacc
2581 ggatccaacg tgttccagac ccgcgccgga tgcctgatcg gagctgagca cgtgaacaat
2641 tcctacgagt gcgacatccc aatcggagct ggaatctgcg ccagctacca gacccagacc
2701 aactccccaa ggagggctcg cagcgtggcc agccagtcca tcatcgccta caccatgtcc
2761 ctgggcgccg agaatagcgt ggcctacagc aacaattcca tcgccatccc aaccaacttc
2821 accatctccg tgaccaccga gatcctgccc gtgtccatga ccaagaccag cgtggactgc
2881 accatgtaca tctgcggcga ttccaccgag tgcagcaacc tgctgctgca gtacggcagc
2941 ttctgcaccc agctgaatcg cgccctgacc ggaatcgctg tggagcagga taagaacacc
3001 caggaggtgt tcgcccaggt gaagcagatc tacaagaccc ccccaatcaa ggacttcggc
3061 ggcttcaatt tcagccagat cctgcccgat ccaagcaagc cctccaagcg cagcttcatc
3121 gaggacctgc tgttcaacaa ggtgaccctg gccgatgccg gcttcatcaa gcagtacggc
3181 gattgcctgg gcgacatcgc tgcccgcgac ctgatctgcg cccagaagtt caatggcctg
3241 accgtgctgc caccactgct gaccgatgag atgatcgctc agtacacctc cgccctgctg
3301 gccggaacca tcaccagcgg atggaccttc ggcgctggag ccgccctgca gatccccttc
3361 gccatgcaga tggcctaccg cttcaacggc atcggcgtga cccagaatgt gctgtacgag
3421 aaccagaagc tgatcgccaa tcagttcaac tccgccatcg gcaagatcca ggactccctg
3481 tccagcaccg ccagcgccct gggcaagctg caggatgtgg tgaatcagaa cgcccaggcc
3541 ctgaataccc tggtgaagca gctgtccagc aacttcggcg ccatctccag cgtgctgaat
3601 gatatcctga gccgcctgga caaggtggag gctgaggtgc agatcgatag gctgatcacc
3661 ggccgcctgc agtccctgca gacctacgtg acccagcagc tgatcagggc tgctgagatc
3721 agggccagcg ccaatctggc tgctaccaag atgtccgagt gcgtgctggg acagagcaag
3781 agggtggact tctgcggcaa gggctaccac ctgatgtcct tcccacagag cgccccacac
3841 ggagtggtgt tcctgcacgt gacctacgtg ccagcccagg agaagaactt caccaccgct
3901 ccagctatct gccacgatgg caaggctcac ttcccacgcg agggcgtgtt cgtgtccaac
3961 ggcacccact ggttcgtgac ccagcgcaat ttctacgagc cccagatcat caccaccgac
4021 aataccttcg tgagcggcaa ctgcgacgtg gtcatcggaa tcgtgaacaa taccgtgtac
4081 gatcccctgc agccagagct ggactccttc aaggaggagc tggataagta cttcaagaat
4141 cacaccagcc ccgacgtgga tctgggcgac atctccggca tcaatgccag cgtggtgaac
4201 atccagaagg agatcgaccg cctgaacgag gtggccaaga atctgaacga gtccctgatc
4261 gatctgcagg agctgggcaa gtacgagcag tacatcaagt ggccatggta catctggctg
4321 ggcttcatcg ccggcctgat cgccatcgtg atggtgacca tcatgctgtg ctgcatgacc
4381 tcctgctgca gctgcctgaa gggctgctgc tcctgcggca gctgctgcta ggaattcctc
4441 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
4501 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
4561 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
4621 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
4681 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
4741 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
4801 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
4861 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
4921 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
4981 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
5041 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
5101 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
5161 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
5221 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
5281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
5341 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
5401 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
5461 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
5521 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
5581 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
5641 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
5701 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
5761 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
5821 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
5881 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
5941 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
6001 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
6061 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
6121 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
6181 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
6241 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
6301 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
6361 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
6421 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
6481 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
6541 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
6601 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
6661 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
6721 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
6781 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
6841 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
6901 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
6961 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
7021 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
7081 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
7141 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
7201 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
7261 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
7321 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
7381 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
7441 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
7501 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
7561 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
7621 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
7681 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
7741 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
7801 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
7861 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
7921 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
7981 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
8041 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
8101 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
8161 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
8221 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
8281 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
8341 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
8401 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
8461 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
8521 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
8581 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
8641 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
8701 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
8761 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
8821 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
8881 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
8941 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
9001 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
9061 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
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